Periplaneta americana arginine kinase as a major cockroach allergen among Thai patients with major cockroach allergies.

ABSTRACT: Periplaneta americana is the predominant cockroach (CR) species and a major source of indoor allergens in Thailand. Nevertheless, data on the nature and molecular characteristics of its allergenic components are rare. We conducted this study to identify and characterize the P. americana allergenic protein. A random heptapeptide phage display library and monoclonal antibody (MAb) specific to a the P. americana component previously shown to be an allergenic molecule were used to identify the MAb-bound mimotope and its phylogenic distribution. Two-dimensional gel electrophoresis, liquid chromatography, mass spectrometry, peptide mass fingerprinting, and BLAST search were used to identify the P. americana protein containing the MAb-specific epitope. We studied the allergenicity of the native p

SUBMITTER: Sookrung N 

PROVIDER: S-EPMC1480524 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature