Role of transcription factor Sp1 and CpG methylation on the regulation of the human podocalyxin gene promoter.

ABSTRACT:

Background

Podocalyxin (podxl) is a heavily glycosylated transmembrane protein mainly found on the apical membrane of rat podocytes and also in endothelial, hematopoietic, and tumor cells. Despite of its interest no much is known about the transcriptional regulation of podxl in different cells. Thus, we aimed at studying the functional features of the 5'-regulatory region of the human Podxl gene.

Results

The promoter region of the human Podxl gene has been cloned and its structure and function were analyzed. The primary DNA sequence is rich in G+C and is devoid of TATA or CAAT boxes. The sequence contains recognition sites for several putative transcription factors; however, the basic promoter activity seems to rely entirely on Sp1 transcription factor since supershift analy

SUBMITTER: Butta N 

PROVIDER: S-EPMC1481587 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature