KSHV targets multiple leukocyte lineages during long-term productive infection in NOD/SCID mice.

ABSTRACT: To develop an animal model of Kaposi sarcoma-associated herpesvirus (KSHV) infection uniquely suited to evaluate longitudinal patterns of viral gene expression, cell tropism, and immune responses, we injected NOD/SCID mice intravenously with purified virus and measured latent and lytic viral transcripts in distal organs over the subsequent 4 months. We observed sequential escalation of first latent and then lytic KSHV gene expression coupled with electron micrographic evidence of virion production within the murine spleen. Using novel technology that integrates flow cytometry with immunofluorescence microscopy, we found that the virus establishes infection in murine B cells, macrophages, NK cells, and, to a lesser extent, dendritic cells. To investigate the potential for human KSHV-specifi

SUBMITTER: Parsons CH 

PROVIDER: S-EPMC1481659 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature