Derivation of species-specific hybridization-like knowledge out of cross-species hybridization results.

ABSTRACT:

Background

One of the approaches for conducting genomics research in organisms without extant microarray platforms is to profile their expression patterns by using Cross-Species Hybridization (CSH). Several different studies using spotted microarray and CSH produced contradicting conclusions in the ability of CSH to reflect biological processes described by species-specific hybridization (SSH).

Results

We used a tomato-spotted cDNA microarray to examine the ability of CSH to reflect SSH data. Potato RNA was hybridized to spotted cDNA tomato and potato microarrays to generate CSH and SSH data, respectively. Difficulties arose in obtaining transcriptomic data from CSH that reflected those obtained from SSH. Nevertheless, once the data was filtered for those corresponding to ma

SUBMITTER: Bar-Or C 

PROVIDER: S-EPMC1482311 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature