Accumulation of small murine minor satellite transcripts leads to impaired centromeric architecture and function.

ABSTRACT: RNAs have been implicated in the assembly and stabilization of large-scale chromatin structures including centromeric architecture; unidentified RNAs are integral components of human pericentric heterochromatin and are required for localization of the heterochromatin protein HP1 to centromeric regions. Because satellite repeats in centromeric regions are known to be transcribed, we assessed a role for noncoding centromeric RNAs in the structure and function of the centromere. We identified minor satellite transcripts of 120 nt in murine cells that localize to centromeres and accumulate upon stress or differentiation. Forced accumulation of 120-nt transcripts leads to defects in chromosome segregation and sister-chromatid cohesion, changes in hallmark centromeric epigenetic markers, and mis

SUBMITTER: Bouzinba-Segard H 

PROVIDER: S-EPMC1482643 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature