Ontogenetic variations in the venom proteome of the Amazonian snake Bothrops atrox.

ABSTRACT:

Background

Bothrops atrox is responsible for the majority of snakebite accidents in the Brazilian Amazon region. Previous studies have demonstrated that the biological and pharmacological activities of B. atrox venom alter with the age of the animal. Here, we present a comparative proteome analysis of B. atrox venom collected from specimens of three different stages of maturation: juveniles, sub-adults and adults.

Results

Optimized conditions for two-dimensional gel electrophoresis (2-DE) of pooled venom samples were achieved using immobilized pH gradient (IPG) gels of non-linear 3-10 pH range during the isoelectric focusing step and 10-20% gradient polyacrylamide gels in the second dimension. Software-assisted analysis of the 2-DE gels images demonstrated differences in the

SUBMITTER: Guercio RA 

PROVIDER: S-EPMC1483819 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature