Using pyrosequencing to shed light on deep mine microbial ecology.

ABSTRACT:

Background

Contrasting biological, chemical and hydrogeological analyses highlights the fundamental processes that shape different environments. Generating and interpreting the biological sequence data was a costly and time-consuming process in defining an environment. Here we have used pyrosequencing, a rapid and relatively inexpensive sequencing technology, to generate environmental genome sequences from two sites in the Soudan Mine, Minnesota, USA. These sites were adjacent to each other, but differed significantly in chemistry and hydrogeology.

Results

Comparisons of the microbes and the subsystems identified in the two samples highlighted important differences in metabolic potential in each environment. The microbes were performing distinct biochemistry on the available

SUBMITTER: Edwards RA 

PROVIDER: S-EPMC1483832 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature