Human monoclonal antibody combination against SARS coronavirus: synergy and coverage of escape mutants.

ABSTRACT:

Background

Experimental animal data show that protection against severe acute respiratory syndrome coronavirus (SARS-CoV) infection with human monoclonal antibodies (mAbs) is feasible. For an effective immune prophylaxis in humans, broad coverage of different strains of SARS-CoV and control of potential neutralization escape variants will be required. Combinations of virus-neutralizing, noncompeting mAbs may have these properties.

Methods and findings

Human mAb CR3014 has been shown to completely prevent lung pathology and abolish pharyngeal shedding of SARS-CoV in infected ferrets. We generated in vitro SARS-CoV variants escaping neutralization by CR3014, which all had a single P462L mutation in the glycoprotein spike (S) of the escape virus. In vitro experiments confirmed

SUBMITTER: ter Meulen J 

PROVIDER: S-EPMC1483912 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature