Gdt1, a new signal transduction component for negative regulation of the growth-differentiation transition in Dictyostelium discoideum.

ABSTRACT: Discoidin I expression was used as a marker to screen for mutants affected in the growth-differentiation transition (GDT) of Dictyostelium. By REMI mutagenesis we have isolated mutant 2-9, an overexpressor of discoidin I. It displays normal morphogenesis but shows premature entry into the developmental cycle. The disrupted gene was denominated gdt1. The mutant phenotype was reconstructed by disruptions in different parts of the gene, suggesting that all had a complete loss of function. gdt1 was expressed in growing cells; the levels of protein and mRNA appear to increase with cell density and rapidly decrease with the onset of development. gdt1 encodes a 175-kDa protein with four putative transmembrane domains. In the C terminus, the derived amino acid sequence displays some similarity to

SUBMITTER: Zeng C 

PROVIDER: S-EPMC14872 | biostudies-literature | 2000 May

REPOSITORIES: biostudies-literature