Microarray analysis of mRNA levels from RAW264.7 macrophages infected with Brucella abortus.

ABSTRACT: Identification of host responses at the gene transcription level provides a molecular profile of the events that occur following infection. Brucella abortus is a facultative intracellular pathogen of macrophages that induces chronic infection in humans and domestic animals. Using microarray technology, the response of macrophages 4 h following B. abortus infection was analyzed to identify early intracellular infection events that occur in macrophages. Of the >6,000 genes, we identified over 140 genes that were reproducibly differentially transcribed. First, an increase in the transcription of a number of proinflammatory cytokines and chemokines, such as tumor necrosis factor alpha, interleukin-1beta (IL-1beta), IL-1alpha, and members of the SCY family of proteins, that may constitute a gen

SUBMITTER: Eskra L 

PROVIDER: S-EPMC148819 | biostudies-literature | 2003 Mar

REPOSITORIES: biostudies-literature