Microarray-based analysis of subnanogram quantities of microbial community DNAs by using whole-community genome amplification.

ABSTRACT: Microarray technology provides the opportunity to identify thousands of microbial genes or populations simultaneously, but low microbial biomass often prevents application of this technology to many natural microbial communities. We developed a whole-community genome amplification-assisted microarray detection approach based on multiple displacement amplification. The representativeness of amplification was evaluated using several types of microarrays and quantitative indexes. Representative detection of individual genes or genomes was obtained with 1 to 100 ng DNA from individual or mixed genomes, in equal or unequal abundance, and with 1 to 500 ng community DNAs from groundwater. Lower concentrations of DNA (as low as 10 fg) could be detected, but the lower template concentrations affect

SUBMITTER: Wu L 

PROVIDER: S-EPMC1489353 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature