Illustration of a common framework for relating multiple typing methods by application to macrolide-resistant Streptococcus pyogenes.

ABSTRACT: The studies that correlate the results obtained by different typing methodologies rely solely on qualitative comparisons of the groups defined by each methodology. We propose a framework of measures for the quantitative assessment of correspondences between different typing methods as a first step to the global mapping of type equivalences. A collection of 325 macrolide-resistant Streptococcus pyogenes isolates associated with pharyngitis cases in Portugal was used to benchmark the proposed measures. All isolates were characterized by macrolide resistance phenotyping, T serotyping, emm sequence typing, and pulsed-field gel electrophoresis (PFGE), using SmaI or Cfr9I and SfiI. A subset of 41 isolates, representing each PFGE cluster, was also characterized by multilocus sequence typing (MLST

SUBMITTER: Carrico JA 

PROVIDER: S-EPMC1489512 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature