Identification of the leucine-to-2-methylbutyric acid catabolic pathway of Lactococcus lactis.

ABSTRACT: Nutrient starvation and nonculturability in bacteria lead to changes in metabolism not found during the logarithmic phase. Substrates alternate to those used during growth are metabolized in these physiological states, yielding secondary metabolites. In firmicutes and actinobacteria, amino acid catabolic pathways are induced during starvation and nonculturability. Examination of lactococci showed that the population entered a nonculturable state after carbohydrate depletion and was incapable of growth on solid media; however, the cells gained the ability to produce branched-chain fatty acids from amino acids. Gene expression profiling and in silico pathway analysis coupled with nuclear magnetic resonance spectroscopy were used to delineate the leucine catabolic pathway. Lactococci produced

SUBMITTER: Ganesan B 

PROVIDER: S-EPMC1489675 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature