CDNA microarray analysis of host-pathogen interactions in a porcine in vitro model for Toxoplasma gondii infection.

ABSTRACT: Toxoplasma gondii induces the expression of proinflammatory cytokines, reorganizes organelles, scavenges nutrients, and inhibits apoptosis in infected host cells. We used a cDNA microarray of 420 annotated porcine expressed sequence tags to analyze the molecular basis of these changes at eight time points over a 72-hour period in porcine kidney epithelial (PK13) cells infected with T. gondii. A total of 401 genes with Cy3 and Cy5 spot intensities of >/=500 were selected for analysis, of which 263 (65.6%) were induced >/=2-fold (expression ratio, >/=2.0; P

SUBMITTER: Okomo-Adhiambo M 

PROVIDER: S-EPMC1489723 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature