Cortactin is a component of clathrin-coated pits and participates in receptor-mediated endocytosis.

ABSTRACT: The actin cytoskeleton is believed to contribute to the formation of clathrin-coated pits, although the specific components that connect actin filaments with the endocytic machinery are unclear. Cortactin is an F-actin-associated protein, localizes within membrane ruffles in cultured cells, and is a direct binding partner of the large GTPase dynamin. This direct interaction with a component of the endocytic machinery suggests that cortactin may participate in one or several endocytic processes. Therefore, the goal of this study was to test whether cortactin associates with clathrin-coated pits and participates in receptor-mediated endocytosis. Morphological experiments with either anti-cortactin antibodies or expressed red fluorescence protein-tagged cortactin revealed a striking colocaliz

SUBMITTER: Cao H 

PROVIDER: S-EPMC149460 | biostudies-literature | 2003 Mar

REPOSITORIES: biostudies-literature