Mixed-genome microarrays reveal multiple serotype and lineage-specific differences among strains of Listeria monocytogenes.

ABSTRACT: Epidemiological studies and analysis of putative virulence genes have shown that Listeria monocytogenes has diverged into several phylogenetic divisions. We hypothesize that similar divergence has occurred for many genes that influence niche-specific fitness and virulence and that identifying these differences may offer new opportunities for the detection, treatment, and control of this important pathogen. To explore this issue further, we developed a microarray composed of fragmented DNA taken from 10 strains of L. monocytogenes. We then hybridized genomic DNA from 50 different strains to replicate arrays and analyzed the resulting hybridization patterns. A simple Euclidean distance metric permitted the reconstruction of previously described genetic relationships between serotypes, and on

SUBMITTER: Call DR 

PROVIDER: S-EPMC149708 | biostudies-literature | 2003 Feb

REPOSITORIES: biostudies-literature