Characterization of the Streptomyces sp. strain C5 snp locus and development of snp-derived expression vectors.

ABSTRACT: The Streptomyces sp. strain C5 snp locus is comprised of two divergently oriented genes: snpA, a metalloproteinase gene, and snpR, which encodes a LysR-like activator of snpA transcription. The transcriptional start point of snpR is immediately downstream of a strong T-N(11)-A inverted repeat motif likely to be the SnpR binding site, while the snpA transcriptional start site overlaps the ATG start codon, generating a leaderless snpA transcript. By using the aphII reporter gene of pIJ486 as a reporter, the plasmid-borne snpR-activated snpA promoter was ca. 60-fold more active than either the nonactivated snpA promoter or the melC1 promoter of pIJ702. The snpR-activated snpA promoter produced reporter protein levels comparable to those of the up-mutated ermE* promoter. The SnpR-activated snp

SUBMITTER: DeSanti CL 

PROVIDER: S-EPMC150044 | biostudies-literature | 2003 Mar

REPOSITORIES: biostudies-literature