Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR.

ABSTRACT: In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

SUBMITTER: Heller LC 

PROVIDER: S-EPMC150052 | biostudies-literature | 2003 Mar

REPOSITORIES: biostudies-literature