Production and utilization of a high-density oligonucleotide microarray in channel catfish, Ictalurus punctatus.

ABSTRACT:

Background

Functional analysis of the catfish genome will be useful for the identification of genes controlling traits of economic importance, especially innate disease resistance. However, this species lacks a platform for global gene expression profiling, so we designed a first generation high-density oligonucleotide microarray platform based on channel catfish EST sequences. This platform was used to profile gene expression in catfish spleens 2 h, 4 h, 8 h and 24 h after injection of lipopolysaccharide (LPS).

Results

In the spleen samples, 138 genes were significantly induced or repressed greater than 2-fold by LPS treatment. Real-time RT-PCR was used to verify the microarray results for nine selected genes representing different expression levels. The results from real-t

SUBMITTER: Li RW 

PROVIDER: S-EPMC1501020 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature