Rapid chemokinetic movement and the invasive potential of lung cancer cells; a functional molecular study.

ABSTRACT:

Background

Non-small cell lung cancer is the most common cause of early casualty from malignant disease in western countries. The heterogeneous nature of these cells has been identified by histochemical and microarray biomarker analyses. Unfortunately, the morphological, molecular and biological variation within cell lines used as models for invasion and metastasis are not well understood. In this study, we test the hypothesis that heterogeneous cancer cells exhibit variable motility responses such as chemokinesis and chemotaxis that can be characterized molecularly.

Methods

A subpopulation of H460 lung cancer cells called KINE that migrated under chemokinetic (no gradient) conditions was harvested from Boyden chambers and cultured. Time-lapsed microscopy, immunofluorescence

SUBMITTER: Tchou-Wong KM 

PROVIDER: S-EPMC1501041 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature