Analysis of prolactin-modulated gene expression profiles during the Nb2 cell cycle using differential screening techniques.

ABSTRACT:

Background

Rat Nb2-11C lymphoma cells are dependent on prolactin for proliferation and are widely used to study prolactin signaling pathways. To investigate the role of this hormone in the transcriptional mechanisms that underlie prolactin-stimulated mitogenesis, five different techniques were used to isolate differentially expressed transcripts: mRNA differential display, representational difference analysis (RDA), subtractive suppressive hybridization (SSH), analysis of weakly expressed candidate genes, and differential screening of an organized library.

Results

About 70 transcripts were found to be modulated in Nb2 cells following prolactin treatment. Of these, approximately 20 represent unknown genes. All cDNAs were characterized by northern blot analysis and categorized

SUBMITTER: Bole-Feysot C 

PROVIDER: S-EPMC15026 | biostudies-literature | 2000

REPOSITORIES: biostudies-literature