A nonerythroid isoform of protein 4.1R interacts with components of the contractile apparatus in skeletal myofibers.

ABSTRACT: The approximately 80-kDa erythroid 4.1R protein is a major component of the erythrocyte cytoskeleton, where it links transmembrane proteins to the underlying spectrin/actin complexes. A diverse collection of 4.1R isoforms has been described in nonerythroid cells, ranging from approximately 30 to approximately 210 kDa. In the current study, we identified the number and primary structure of 4.1R isoforms expressed in adult skeletal muscle and characterized the localization patterns of 4.1R message and protein. Skeletal muscle 4.1R appears to originate solely from the upstream translation initiation codon (AUG-1) residing in exon 2'. Combinations of alternatively spliced downstream exons generate an array of distinct 4.1R spliceoforms. Two major isoform classes of approximately 105/110 and ap

SUBMITTER: Kontrogianni-Konstantopoulos A 

PROVIDER: S-EPMC15038 | biostudies-literature | 2000 Nov

REPOSITORIES: biostudies-literature