Radiation hybrid mapping of cataract genes in the dog.

ABSTRACT:

Purpose

To facilitate the molecular characterization of naturally occurring cataracts in dogs by providing the radiation hybrid location of 21 cataract-associated genes along with their closely associated polymorphic markers. These can be used for segregation testing of the candidate genes in canine cataract pedigrees.

Methods

Twenty-one genes with known mutations causing hereditary cataracts in man and/or mouse were selected and mapped to canine chromosomes using a canine:hamster radiation hybrid RH5000 panel. Each cataract gene ortholog was mapped in relation to over 3,000 markers including microsatellites, ESTs, genes, and BAC clones. The resulting independently determined RH-map locations were compared with the corresponding gene locations from the draft sequence of the

SUBMITTER: Hunter LS 

PROVIDER: S-EPMC1509099 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature