Transcriptome analysis of Sinorhizobium meliloti during symbiosis.

ABSTRACT: BACKGROUND:Rhizobia induce the formation on specific legumes of new organs, the root nodules, as a result of an elaborated developmental program involving the two partners. In order to contribute to a more global view of the genetics underlying this plant-microbe symbiosis, we have mined the recently determined Sinorhizobium meliloti genome sequence for genes potentially relevant to symbiosis. We describe here the construction and use of dedicated nylon macroarrays to study simultaneously the expression of 200 of these genes in a variety of environmental conditions, pertinent to symbiosis. RESULTS:The expression of 214 S. meliloti genes was monitored under ten environmental conditions, including free-living aerobic and microaerobic conditions, addition of the plant symbiotic elicitor luteo

SUBMITTER: Ampe F 

PROVIDER: S-EPMC151305 | biostudies-literature | 2003

REPOSITORIES: biostudies-literature