Transcriptional profiling of mesenchymal stromal cells from young and old rats in response to Dexamethasone.

ABSTRACT:

Background

Marrow-derived stromal cells (MSCs) maintain the capability of self-renewal and differentiation into multiple lineages in adult life. Age-related changes are recognized by a decline in the stemness potential that result in reduced regeneration potential of the skeleton. To explore the molecular events that underline skeletal physiology during aging we catalogued the profile of gene expression in ex vivo cultured MSCs derived from 3 and 15 month old rats. The ex vivo cultured cells were analyzed following challenge with or without Dexamethasone (Dex). RNA retrieved from these cells was analyzed using Affymetrix Gene Chips to compare the effect of Dex on gene expression in both age groups.

Results

The molecular mechanisms that underline skeletal senescence were stud

SUBMITTER: Akavia UD 

PROVIDER: S-EPMC1513212 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature