Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay.

ABSTRACT:

Background

The classification of Brucella into species and biovars relies on phenotypic characteristics and sometimes raises difficulties in the interpretation of the results due to an absence of standardization of the typing reagents. In addition, the resolution of this biotyping is moderate and requires the manipulation of the living agent. More efficient DNA-based methods are needed, and this work explores the suitability of multiple locus variable number tandem repeats analysis (MLVA) for both typing and species identification.

Results

Eighty tandem repeat loci predicted to be polymorphic by genome sequence analysis of three available Brucella genome sequences were tested for polymorphism by genotyping 21 Brucella strains (18 reference strains representing the six 'class

SUBMITTER: Le Fleche P 

PROVIDER: S-EPMC1513380 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature