Genetic diversity of Clostridium perfringens type A isolates from animals, food poisoning outbreaks and sludge.

ABSTRACT:

Background

Clostridium perfringens, a serious pathogen, causes enteric diseases in domestic animals and food poisoning in humans. The epidemiological relationship between C. perfringens isolates from the same source has previously been investigated chiefly by pulsed-field gel electrophoresis (PFGE). In this study the genetic diversity of C. perfringens isolated from various animals, from food poisoning outbreaks and from sludge was investigated.

Results

We used PFGE to examine the genetic diversity of 95 C. perfringens type A isolates from eight different sources. The isolates were also examined for the presence of the beta2 toxin gene (cpb2) and the enterotoxin gene (cpe). The cpb2 gene from the 28 cpb2-positive isolates was also partially sequenced (519 bp, corresponding t

SUBMITTER: Johansson A 

PROVIDER: S-EPMC1513381 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature