Analysis of binding at a single spatially localized cluster of binding sites by fluorescence recovery after photobleaching.

ABSTRACT: Cells contain many subcellular structures in which specialized proteins locally cluster. Binding interactions within such clusters may be analyzed in live cells using models for fluorescence recovery after photobleaching (FRAP). Here we analyze a three-dimensional FRAP model that accounts for a single spatially localized cluster of binding sites in the presence of both diffusion and impermeable boundaries. We demonstrate that models completely ignoring the spatial localization of binding yield poor estimates for the binding parameters within the binding site cluster. In contrast, we find that ignoring only the restricted axial height of the binding-site cluster is far less detrimental, thereby enabling the use of computationally less expensive models. We also identify simplified solutions

SUBMITTER: Sprague BL 

PROVIDER: S-EPMC1518639 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature