Novel functions of the phosphatidylinositol metabolic pathway discovered by a chemical genomics screen with wortmannin.

ABSTRACT: We report a novel connection between the phosphatidylinositol (PI) metabolic pathway and the DNA replication and damage checkpoint pathway discovered from an unbiased chemical genomics screen. Substrates and products of PI kinases are important signaling molecules that affect a wide range of biological processes. The full collection of yeast deletion strains was screened to identify genes that confer altered sensitivity to the natural product wortmannin, a PI kinase inhibitor. These experiments have allowed us to explore metabolomic and proteomic implications of PI synthesis and turnover. This study also uncovers other biological processes affected by wortmannin treatment, including proteasome-mediated degradation and chromatin remodeling. Bioinformatic analyses were used to reveal the rel

SUBMITTER: Zewail A 

PROVIDER: S-EPMC152295 | biostudies-literature | 2003 Mar

REPOSITORIES: biostudies-literature