Expression of target and reference genes in Daphnia magna exposed to ibuprofen.

ABSTRACT:

Background

Transcriptomic techniques are now being applied in ecotoxicology and toxicology to measure the impact of stressors and develop understanding of mechanisms of toxicity. Microarray technology in particular offers the potential to measure thousands of gene responses simultaneously. However, it is important that microarrays responses should be validated, at least initially, using real-time quantitative polymerase chain reaction (QPCR). The accurate measurement of target gene expression requires normalisation to an invariant internal control e.g., total RNA or reference genes. Reference genes are preferable, as they control for variation inherent in the cDNA synthesis and PCR. However, reference gene expression can vary between tissues and experimental conditions, which makes

SUBMITTER: Heckmann LH 

PROVIDER: S-EPMC1523214 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature