Identification of rat lung--prominent genes by a parallel DNA microarray hybridization.

ABSTRACT:

Background

The comparison of organ transcriptomes is an important strategy for understanding gene functions. In the present study, we attempted to identify lung-prominent genes by comparing the normal transcriptomes of rat lung, heart, kidney, liver, spleen, and brain. To increase the efficiency and reproducibility, we first developed a novel parallel hybridization system, in which 6 samples could be hybridized onto a single slide at the same time.

Results

We identified the genes prominently expressed in the lung (147) or co-expressed in lung-heart (23), lung-liver (37), lung-spleen (203), and lung-kidney (98). The known functions of the lung-prominent genes mainly fell into 5 categories: ligand binding, signal transducer, cell communication, development, and metabolism. Rea

SUBMITTER: Chen Z 

PROVIDER: S-EPMC1523215 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature