Biologically relevant effects of mRNA amplification on gene expression profiles.

ABSTRACT:

Background

Gene expression microarray technology permits the analysis of global gene expression profiles. The amount of sample needed limits the use of small excision biopsies and/or needle biopsies from human or animal tissues. Linear amplification techniques have been developed to increase the amount of sample derived cDNA. These amplified samples can be hybridised on microarrays. However, little information is available whether microarrays based on amplified and unamplified material yield comparable results. In the present study we compared microarray data obtained from amplified mRNA derived from biopsies of rat cardiac left ventricle and non-amplified mRNA derived from the same organ. Biopsies were linearly amplified to acquire enough material for a microarray experiment. Both

SUBMITTER: van Haaften RI 

PROVIDER: S-EPMC1523219 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature