TF Target Mapper: a BLAST search tool for the identification of Transcription Factor target genes.

ABSTRACT:

Background

In the current era of high throughput genomics a major challenge is the genome-wide identification of target genes for specific transcription factors. Chromatin immunoprecipitation (ChIP) allows the isolation of in vivo binding sites of transcription factors and provides a powerful tool for examining gene regulation. Crosslinked chromatin is immunoprecipitated with antibodies against specific transcription factors, thus enriching for sequences bound in vivo by these factors in the immunoprecipitated DNA. Cloning and sequencing the immunoprecipitated sequences allows identification of transcription factor target genes. Routinely, thousands of such sequenced clones are used in BLAST searches to map their exact location in the genome and the genes located in the vicinity. T

SUBMITTER: Horsman S 

PROVIDER: S-EPMC1523221 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature