PCR-oligonucleotide ligation assay for detection of point mutations associated with quinolone resistance in Streptococcus pneumoniae.

ABSTRACT: We have developed a PCR-oligonucleotide ligation assay to rapidly identify base substitutions in topoisomerase genes that are associated with quinolone resistance in clinical isolates of Streptococcus pneumoniae. Thirty-seven strains for which the ciprofloxacin MICs were >/=4 micro g/ml and 16 strains for which the MICs were

SUBMITTER: Bui MH 

PROVIDER: S-EPMC152482 | biostudies-literature | 2003 Apr

REPOSITORIES: biostudies-literature