Chromosome-wide identification of novel imprinted genes using microarrays and uniparental disomies.

ABSTRACT: Genomic imprinting refers to a specialized form of epigenetic gene regulation whereby the expression of a given allele is dictated by parental origin. Defining the extent and distribution of imprinting across genomes will be crucial for understanding the roles played by imprinting in normal mammalian growth and development. Using mice carrying uniparental disomies or duplications, microarray screening and stringent bioinformatics, we have developed the first large-scale tissue-specific screen for imprinted gene detection. We quantify the stringency of our methodology and relate it to previous non-tissue-specific large-scale studies. We report the identification in mouse of four brain-specific novel paternally expressed transcripts and an additional three genes that show maternal expression

SUBMITTER: Schulz R 

PROVIDER: S-EPMC1524921 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature