Transcription profiles of non-immortalized breast cancer cell lines.

ABSTRACT:

Background

Searches for differentially expressed genes in tumours have made extensive use of array technology. Most samples have been obtained from tumour biopsies or from established tumour-derived cell lines. Here we compare cultures of non-immortalized breast cancer cells, normal non-immortalized breast cells and immortalized normal and breast cancer cells to identify which elements of a defined set of well-known cancer-related genes are differentially expressed.

Methods

Cultures of cells from pleural effusions or ascitic fluids from breast cancer patients (MSSMs) were used in addition to commercially-available normal breast epithelial cells (HMECs), established breast cancer cell lines (T-est) and established normal breast cells (N-est). The Atlas Human Cancer 1.2 cDNA e

SUBMITTER: Fernandez-Cobo M 

PROVIDER: S-EPMC1524972 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature