AtRTPrimer: database for Arabidopsis genome-wide homogeneous and specific RT-PCR primer-pairs.

ABSTRACT:

Background

Primer design is a critical step in all types of RT-PCR methods to ensure specificity and efficiency of a target amplicon. However, most traditional primer design programs suggest primers on a single template of limited genetic complexity. To provide researchers with a sufficient number of pre-designed specific RT-PCR primer pairs for whole genes in Arabidopsis, we aimed to construct a genome-wide primer-pair database.

Description

We considered the homogeneous physical and chemical properties of each primer (homogeneity) of a gene, non-specific binding against all other known genes (specificity), and other possible amplicons from its corresponding genomic DNA or similar cDNAs (additional information). Then, we evaluated the reliability of our database with selecte

SUBMITTER: Han S 

PROVIDER: S-EPMC1524993 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature