Importance of dosage standardization for interpreting transcriptomal signature profiles: evidence from studies of xenoestrogens.

ABSTRACT: To obtain insights into similarities and differences in the biological actions of related drugs or toxic agents, their transcriptomal signature profiles (TSPs) have been examined in a large number of studies. However, many such reports did not provide proper justification for the dosage criteria of each agent. Using a well characterized cell culture model of estrogen-dependent proliferation of MCF7 human breast cancer cells, we demonstrate how different approaches to dosage standardization exert critical influences on TSPs, leading to different and even conflicting conclusions. Using quantitative cellular response (QCR)-based dosage criteria, TSPs were determined by Affymetrix microarray when cells were proliferating at comparable rates in the presence of various estrogens. We observed tha

SUBMITTER: Shioda T 

PROVIDER: S-EPMC1525050 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature