Identical probes on different high-density oligonucleotide microarrays can produce different measurements of gene expression.

ABSTRACT:

Background

There are many potential sources of variability in a microarray experiment. Variation can arise from many aspects of the collection and processing of samples for gene expression analysis. Oligonucleotide-based arrays are thought to minimize one source of variability as identical oligonucleotides are expected to recognize the same transcripts during hybridization.

Results

We demonstrate that although the probes on the U133A GeneChip arrays are identical in sequence to probes designed for the U133 Plus 2.0 arrays the values obtained from an experimental hybridization can be quite different. Nearly half of the probesets in common between the two array types can produce slightly different values from the same sample. Nearly 70% of the individual probes in these probes

SUBMITTER: Zhang L 

PROVIDER: S-EPMC1525186 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature