Targeting of transmembrane protein shrew-1 to adherens junctions is controlled by cytoplasmic sorting motifs.

ABSTRACT: We recently identified transmembrane protein shrew-1 and showed that it is able to target to adherens junctions in polarized epithelial cells. This suggested shrew-1 possesses specific basolateral sorting motifs, which we analyzed by mutational analysis. Systematic mutation of amino acids in putative sorting signals in the cytoplasmic domain of shrew-1 revealed three tyrosines and a dileucine motif necessary for basolateral sorting. Substitution of these amino acids leads to apical localization of shrew-1. By applying tannic acid to either the apical or basolateral part of polarized epithelial cells, thereby blocking vesicle fusion with the plasma membrane, we obtained evidence that the apically localized mutants were primarily targeted to the basolateral membrane and were then redistribut

SUBMITTER: Jakob V 

PROVIDER: S-EPMC1525240 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature