Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins.

ABSTRACT:

Background

Trisomy of human chromosome 21 (Chr21) results in Down's syndrome, a complex developmental and neurodegenerative disease. Molecular analysis of Down's syndrome, however, poses a particular challenge, because the aneuploid region of Chr21 contains many genes of unknown function. Subcellular localization of human Chr21 proteins may contribute to further understanding of the functions and regulatory mechanisms of the genes that code for these proteins. Following this idea, we used a transfected-cell array technique to perform a rapid and cost-effective analysis of the intracellular distribution of Chr 21 proteins.

Results

We chose 89 genes that were distributed over the majority of 21q, ranging from RBM11 (14.5 Mb) to MCM3AP (46.6 Mb), with part of them expressed abe

SUBMITTER: Hu YH 

PROVIDER: S-EPMC1526728 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature