Real-Time PCR for detection of herpes simplex virus without nucleic acid extraction.

ABSTRACT:

Background

The speed and sensitivity of real-time polymerase chain reaction (PCR) have made it a popular method for the detection of microbiological agents in both research and clinical specimens. For the detection and genotyping of herpes simplex virus (HSV) in clinical specimens, real-time PCR has proven to be faster, more sensitive and safer than earlier methods which included isolation of the virus in cell culture followed by immunofluorescence microscopy. While PCR-based assays for HSV detection posses clear advantages over these earlier techniques, certain aspects of the PCR method remain onerous. The process of extraction and purification of nucleic acid from clinical specimens prior to PCR is particularly cumbersome. Nucleic acid extraction is expensive, time-consuming and

SUBMITTER: Pandori MW 

PROVIDER: S-EPMC1533833 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature