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Molecular detection of Histoplasma capsulatum var. capsulatum in human clinical samples.

ABSTRACT: We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens.

SUBMITTER: Bracca A 

PROVIDER: S-EPMC153865 | biostudies-literature | 2003 Apr

REPOSITORIES: biostudies-literature

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Molecular detection of Histoplasma capsulatum var. capsulatum in human clinical samples.

Bracca Andrea A   Tosello María Elena ME   Girardini Javier E JE   Amigot Susana L SL   Gomez Carlos C   Serra Esteban E  

Journal of clinical microbiology 20030401 4

We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens. ...[more]

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