Transactivation of elements in the human endogenous retrovirus W family by viral infection.

ABSTRACT:

Background

Aberrant expression of human endogenous retrovirus (HERV) elements in the W family has previously been associated with schizophrenia, multiple sclerosis and preeclampsia. Little is know regarding the basal expression, transcriptional regulation and functional significance of individual HERV-elements. Since viral infections have previously been reported to transactivate retroviral long terminal repeat regions we examined the basal expression of HERV-W elements and following infections by influenza A/WSN/33 and Herpes simplex 1 viruses in human cell-lines.

Methods

Relative levels of transcripts encoding HERV-W elements and cellular genes were analyzed by qPCR methods. An analysis of amplicon melting temperatures was used to detect variations in the frequencies of am

SUBMITTER: Nellaker C 

PROVIDER: S-EPMC1539011 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature