PTcINDEX: a stable tetracycline-regulated expression vector for Trypanosoma cruzi.

ABSTRACT:

Background

Trypanosoma cruzi is a protozoan pathogen of major medical importance in Latin America. It is also an early diverging eukaryote that displays many unusual biochemical features. The completion of the T. cruzi genome project has highlighted the need to extend the range of techniques available to study gene function. To this end we report the development of a stable tetracycline-dependent expression vector applicable to this parasite and describe in detail the parameters of the system.

Results

We first produced T. cruzi cell lines that constitutively expressed bacteriophage T7 RNA polymerase and the tetracycline repressor protein from a multicopy episome. An integrative vector with an inducible expression site under the control of a tetracycline-regulatable T7 promot

SUBMITTER: Taylor MC 

PROVIDER: S-EPMC1544328 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature