Production of native-type Streptoverticillium mobaraense transglutaminase in Corynebacterium glutamicum.

ABSTRACT: We previously observed secretion of active-form transglutaminase in Corynebacterium glutamicum by coexpressing the subtilisin-like protease SAM-P45 from Streptomyces albogriseolus to process the prodomain. However, the N-terminal amino acid sequence of the transglutaminase differed from that of the native Streptoverticillium mobaraense enzyme. In the present work we have used site-directed mutagenesis to generate an optimal SAM-P45 cleavage site in the C-terminal region of the prodomain. As a result, native-type transglutaminase was secreted.

SUBMITTER: Date M 

PROVIDER: S-EPMC154552 | biostudies-literature | 2003 May

REPOSITORIES: biostudies-literature