The identification of markers segregating with resistance to Schistosoma mansoni infection in the snail Biomphalaria glabrata.

ABSTRACT: Both snail and parasite genes determine the susceptibility of the snail Biomphalaria glabrata to infection with the trematode Schistosoma mansoni. To identify molecular markers associated with resistance to the parasite in the snail host, we performed genetic crosses between parasite-resistant and -susceptible isogenic snails. Because resistance to infection in adult snails is controlled by a single locus, DNA samples from individual F2 and F1 backcross progeny, segregating for either the resistant or susceptible phenotypes, were pooled (bulked segregant). Genotypes for both parents were determined with 205 arbitrary decamer primers by random amplified polymorphic DNA-PCR. Of the 205 primers, 144 were informative, and the relative allele frequencies between the pools for these primers were

SUBMITTER: Knight M 

PROVIDER: S-EPMC15498 | biostudies-literature | 1999 Feb

REPOSITORIES: biostudies-literature