High-resolution genomic and expression profiling reveals 105 putative amplification target genes in pancreatic cancer.

ABSTRACT: Comparative genomic hybridization (CGH) studies have provided a wealth of information on common copy number aberrations in pancreatic cancer, but the genes affected by these aberrations are largely unknown. To identify putative amplification target genes in pancreatic cancer, we performed a parallel copy number and expression survey in 13 pancreatic cancer cell lines using a 12,232-clone cDNA microarray, providing an average resolution of 300 kb throughout the human genome. CGH on cDNA microarray allowed highly accurate mapping of copy number increases and resulted in identification of 24 independent amplicons, ranging in size from 130 kb to 11 Mb. Statistical evaluation of gene copy number and expression data across all 13 cell lines revealed a set of 105 genes whose elevated expression l

SUBMITTER: Mahlamaki EH 

PROVIDER: S-EPMC1550328 | biostudies-literature | 2004 Sep-Oct

REPOSITORIES: biostudies-literature