Given the opportunity, the Sendai virus RNA-dependent RNA polymerase could as well enter its template internally.

ABSTRACT: The negative-stranded RNA viral genome is an RNA-protein complex of helicoidal symmetry, resistant to nonionic detergent and high salt, in which the RNA is protected from RNase digestion. The 15,384 nucleotides of the Sendai virus genome are bound to 2,564 subunits of the N protein, each interacting with six nucleotides so tightly that the bases are poorly accessible to soluble reagents. With such a uniform structure, the question of template recognition by the viral RNA polymerase has been raised. In a previous study, the N-phase context has been proposed to be crucial for this recognition, a notion referring to the importance of the position in which the nucleotides interact with the N protein. The N-phase context ruled out the role of the template 3'-OH congruence, a feature resulting f

SUBMITTER: Vulliemoz D 

PROVIDER: S-EPMC155117 | biostudies-literature | 2002 Aug

REPOSITORIES: biostudies-literature