Gene expression in acute Stanford type A dissection: a comparative microarray study.

ABSTRACT:

Background

We compared gene expression profiles in acutely dissected aorta with those in normal control aorta.

Materials and methods

Ascending aorta specimen from patients with an acute Stanford A-dissection were taken during surgery and compared with those from normal ascending aorta from multiorgan donors using the BD Atlas Human1.2 Array I, BD Atlas Human Cardiovascular Array and the Affymetrix HG-U133A GeneChip. For analysis only genes with strong signals of more than 70 percent of the mean signal of all spots on the array were accepted as being expressed. Quantitative real-time polymerase chain reaction (RT-PCR) was used to confirm regulation of expression of a subset of 24 genes known to be involved in aortic structure and function.

Results

According to our defi

SUBMITTER: Weis-Muller BT 

PROVIDER: S-EPMC1557406 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature